Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury.
Gene names
HCNTFCiliary neurotrophic factorCNTF
Antibodie's label
Biotin
Purity
Anti-hCNTF specific antibody was purified by affinity chromatography and then biotinylated.
Ab raised in
Rabbit
Type
Polyclonal
Antigen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCNTF (human CNTF).
Immuno application
ELISA, Western Blot
Antibody advice
ELISA:To detect hCNTF by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 ug/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hCNTF. Sandwich: To detect hCNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human CNTF (XP-5115) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCNTF. Western Blot:To detect hCNTF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCNTF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Keep at
CNTF antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
Works with
Human
Omim nr
116585
Ncbi nr
P26441
Vial with antibody
Lyophilized
NCBI GENE nr
1270
GENE symbol
CNTF
Antigene name
ciliary neurotrophic factor
Source
Homo sapiens
Protein nr
P26441
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
