Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury.
Gene names
Ciliary neurotrophic factorCNTF
Antibodie's label
unlabelled serum
Purity
Anti-rCNTF specific antibody was purified by affinity chromatography employing immobilized rCNTF matrix.
Ab raised in
Rabbit
Type
Polyclonal
Antigen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rCNTF (Rat Ciliary Neurotrophic Factor).
Immuno application
ELISA, Western Blot, NELISAut
Antibody advice
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rCNTF (0.15 ng/mL), a concentration of 0.02 - 0.05 ug/mL of this antibody is required. ELISA:To detect rCNTF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rCNTF. Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat CNTF (XP-5114Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CNTF. Western Blot:To detect rCNTF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rCNTF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Keep at
CNTF antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
Works with
Rat
Omim nr
116587
Ncbi nr
P20294
Vial with antibody
Lyophilized
NCBI GENE nr
25707
GENE symbol
Cntf
Antigene name
ciliary neurotrophic factor
Source
Rattus norvegicustus norvegicus
Protein nr
P20294
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
