Gene info
Neuroserpin is a member of the serpin superfamily and is predominantly expressed by neurons of the developing and adult brain. This serpin is secreted from axonal growth cones of the CNS and PNS where it inhibits the enzyme tissue plasminogen activator. The expression pattern of neuroserpin and its in vivo inhibitory activity implicate this serpin in regulating axonal growth, reducing seizure activity, controlling damage in cerebral infarction and regulating emotional behaviour and memory. Four different mutations have been described in the human neuroserpin gene that promote neuroserpin abnormal polymerization, giving rise to intraneuronal inclusion bodies and a new form of dementia called Familial Encephalopathy with Neuroserpin Inclusion Bodies.
Gene names
PI12, neuroserpin, PI12, Neuroserpin, Peptidase inhibitor 12, PI-12
Antibodie's label
unlabelled serum
Ab raised in
Rabbit
Type
Polyclonal
Antigen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNeuroserpin (human Neuroserpin). Human Neuroserpin specific antibody was purified by affinity chromatography employing immobilized hNeuroserpin matrix.
Immuno application
ELISA, Western Blot
Antibody advice
ELISA:Indirect:To detect hNeuroserpin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNeuroserpin.SandwichTo detect hNeuroserpin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Neuroserpin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNeuroserpin. Western Blot:To detect hNeuroserpin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNeuroserpin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Keep at
Neuroserpin antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
Works with
Human
Omim nr
3183087
Ncbi nr
Q99574
Vial with antibody
Lyophilized
NCBI GENE nr
5274
GENE symbol
SERPINI1
Antigene name
serpin peptidase inhibitor, clade I (neuroserpin), member 1
Source
Homo sapiens
Protein nr
Q99574
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps