A tyrosine kinase receptor in the EGF-receptor family. ErbB2 does not bind ligand on its own, but acts as an amplifier in heterodimers with ErbB1, (EGFR), ErbB3, or ErbB4. Amplification of the ErbB2 gene and overexpression of ErbB2 protein is associated with breast cancer (~25% of cases), and anti-ErbB2 therapeutics (e.g. Herceptin, an anti-ErbB2 monoclonal antibody) are sometimes effective in treating these cases. Overexpression of ErbB2 has also been indicated as a factor in prostate cancer.
BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Antigen
ERBB2 antibody was made against a peptide located in the cytoplasmic domain of ERBB2 (Human).
Immuno application
immuno-histo-chemistry
Antibody advice
ERBB2 antibody can be used in ELISA starting at 1:10000, Western Blot starting at 1:500 - 1:2000, immunohistochemistry starting at 1:200, and immunofluorescence starting at 1:200 - 1:1000.
Suspention, pH, azide
PBS, 0.1% sodium azide.
Keep at
ERBB2 antibody should be stored long term (months) at -80 grades C and short term (days) at 4 grades C. As with all antibodies avoid freeze/thaw cycles.
Works with
Gibbon, Bovine, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Rat
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
