NET1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Ab raised in
Goat
Type
Polyclonal
Spicific to
This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Antigen
NET1 antibody was made against a 14 amino acid synthetic peptide near the C-Terminus of NET1.
Immuno application
ELISA, immuno-histo-chemistry
Antibody advice
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 85 kDa in human placenta and a very strong band at approximately 40-50 kDa in human skeletal muscle lysates after 0.1 ug/mL staining.7 kDa according to NP_001040625.1 and of 61.9 kDa according to NP_005854.2. Immunohistochemistry: In paraffin embedded human tonsil shows staining of fibrous structures. Recommended concentration, 2.5 ug/mL.
Suspention, pH, azide
NET1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Keep at
Aliquot and store at -20 grades C. Minimize freezing and thawing.
Works with
Human
Omim nr
114145479
Ncbi nr
NP_001040625.1, NP_005854.2
Vial with antibody
in solution
Quantity per ml
500 ug/mL
NCBI GENE nr
10276
GENE symbol
NET1
Antigene name
neuroepithelial cell transforming 1
Source
Homo sapiens
Protein nr
Q7Z628
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
