anti-CHRNA4 Antibody

Gene names

neuronal nicotinic acetylcholine receptor alpha-4 subunit, neuronal acetylcholine receptor alpha 4 subunit, cholinergic receptor, nicotinic, alpha polypeptide 4, alpha 4 subunit, NACRA4, EBN1, EBN, BFNC, alpha 4, CHRNA4

Antibodie's label

unlabelled serum

Purity

Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Ab raised in

Goat

Type

Polyclonal

Spicific to

This antibody is expected NOT to cross-react with the similar alpha 2 subunit.

Antigen

CHRNA4 antibody was made against a 15 amino acid synthetic peptide near the internal region of CHRNA4 (aa 29-43).

Immuno application

ELISA, Western Blot

Antibody advice

ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 70 kDa band observed in rat brain lysates (calculated MW of 70.0 kDa according to human NP_000735.1and of 70.2 kDa according to rat NP_077330.1). Recommended concentration: 1-3 ug/mL._x000D_ an additional band of unknown identity was also consistently observed at 22 kDa . This band was successfully blocked by incubation with the immunizing peptide.

Suspention, pH, azide

CHRNA4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.

Keep at

Aliquot and store at -20 grades C. Minimize freezing and thawing.

Works with

Rat

Omim nr

4502827

Ncbi nr

NP_000735.1

Vial with antibody

in solution

Quantity per ml

500 ug/mL

NCBI GENE nr

1137

GENE symbol

CHRNA4

Antigene name

cholinergic receptor, nicotinic, alpha 4 (neuronal)

Source

Homo sapiens

Protein nr

P43681

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

French translation

anticorps