Gene names
Neurogranin, protein kinase C substrate, neurogranin, calmodulin-binding protein, hng, RC3, neurogranin (protein kinase C substrate, RC3), NRGN
Antibodie's label
unlabelled serum
Purity
Neurogranin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Ab raised in
Goat
Type
Polyclonal
Antigen
Neurogranin antibody was made against an 11 amino acid synthetic peptide near the internal region of Neurogranin.
Immuno application
ELISA, Western Blot
Antibody advice
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 15 kDa band observed in human brain (cerebral cortex and hippocampus), and in mouse brain and rat brain lysates (calculated MW of 7.6 kDa according to NP_006167.1). There is also an additional 16 kDa band. The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15;274 (3) :1294-300; PMID: 9880498). Recommended concentration: 0.1-0.3 ug/mL. Immunohistochemistry: In paraffin embedded human cortex shows cytoplasm staining in the neuronal cell bodies Recommended concentration, 4-6 ug/mL.
Suspention, pH, azide
Neurogranin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Keep at
Aliquot and store at -20 grades C. Minimize freezing and thawing.
Works with
Human, Mouse, Rat
Omim nr
5453800
Ncbi nr
NP_006167.1
Vial with antibody
in solution
Quantity per ml
500 ug/mL
NCBI GENE nr
4900
GENE symbol
NRGN
Antigene name
neurogranin (protein kinase C substrate, RC3)
Source
Homo sapiens
Protein nr
Q92686
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps