Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. NRCAM is a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM may also play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of its gene have been associated with autism and addiction vulnerability.Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms.
Gene names
NRCAM, KIAA0343, MGC138845, MGC138846
Antibodie's label
unlabelled serum
Purity
Antibody is purified by protein A chromatography method.
Ab raised in
Rabbit
Type
Polyclonal
Antigen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NRCAM.
Immuno application
ELISA, Western Blot, immuno-histo-chemistry
Antibody advice
NRCAM antibody can be used for detection of NRCAM by ELISA at 1:62500. NRCAM antibody can be used for detection of NRCAM by western blot at 2.5 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Suspention, pH, azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
Keep at
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store NRCAM antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
Works with
Human
Omim nr
81158226
Ncbi nr
NP_001032209
KDa
141 kDa, 131 kDa
Vial with antibody
Lyophilized
Quantity per ml
1 mg/ml
NCBI GENE nr
4897
GENE symbol
NRCAM
Antigene name
neuronal cell adhesion molecule
Source
Homo sapiens
Protein nr
Q92823
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
