The protein encoded by CHRNA7 displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia.
Gene names
CHRNA7, NACHRA7, CHRNA7-2
Antibodie's label
unlabelled serum
Purity
Antibody is purified by protein A chromatography method.
Ab raised in
Rabbit
Type
Polyclonal
Antigen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHRNA7.
Immuno application
ELISA, Western Blot
Antibody advice
CHRNA7 antibody can be used for detection of CHRNA7 by ELISA at 1:62500. CHRNA7 antibody can be used for detection of CHRNA7 by western blot at 1.25 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Suspention, pH, azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
Keep at
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store CHRNA7 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
