Alternative Names
Pimaricin; Natacyn
Assay Type
Competitive Inhibition
Sensitivity
1.45ng/mL
Standard
300ng/mL
Detection range
3.70-300ng/mL
Assay length
2h
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Natamycin (NTM) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Natamycin (NTM) and unlabeled Natamycin (NTM) (Standards or samples) with the pre-coated antibody specific to Natamycin (NTM). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Natamycin (NTM) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Natamycin (NTM) in the sample.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED