Alternative Names
NM-U
Assay Type
Competitive Inhibition
Sensitivity
48.3pg/mL
Standard
10000pg/mL
Detection range
123.46-10000pg/mL
Assay length
2h
Research Area
Endocrinology;Cardiovascular biology;Neuro science;Gastroenterology;Hormone metabolism;Bone metabolism;
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neuromedin U (NMU) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neuromedin U (NMU) and unlabeled Neuromedin U (NMU) (Standards or samples) with the pre-coated antibody specific to Neuromedin U (NMU). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neuromedin U (NMU) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neuromedin U (NMU) in the sample.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED