This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the N-terminal of human BNDF
Swiss-Prot
P23560(Human) P21237(Mouse) P23363(Rat)
Reactivity
Human, Mouse, Rat
Applications
WB ICC IHC
Application_all
WB:1:500 ICC:1:200 IHC:1:200
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity
Peptide affinity purified
Storage&Stability
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity
BDNF polyclonal antibody detects endogenous levels of BDNF protein.
Usage
Western blot analysis of BDNF on different cell lysates using anti-BDNF antibody at 1/500 dilution. Positive control: Lane 1: A172 Lane 2: SHG-44 Lane3: Mouse heart Lane 4: Mouse brain
BiowMW
28kDa
Note
For research use only, not for use in diagnostic procedure.
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
