Alternative Name
Neuropilin-1; Vascular endothelial cell growth factor 165 receptor; CD_antigen=CD304; NRP1; NRP; VEGF165R
Immunogen
Synthetic peptide, corresponding to amino acids 501-550 of Human Neuropilin-1.
Swiss-Prot
O14786
Reactivity
Human,Mouse,Rat
Applications
WB IHC
Application_all
WB: 1:500~1:1000_x000D_ IHC: 1:50~1:200
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity
Neuropilin-1 (D525) polyclonal antibody detects endogenous levels of Neuropilin-1 protein.
Usage
Western blot (WB) analysis of Neuropilin-1 (D525) pAb at 1:1000 dilution Lane1:L02 whole cell lysate(20ug) Lane2:A549 whole cell lysate(20ug) Lane3:PC3 whole cell lysate(20ug) Lane4:The Heart tissue lysate of Rat(40ug) Lane5:The Brain tissue lysate of Mouse(40ug)
BiowMW
~ 130 kDa
Note
For research use only, not for use in diagnostic procedure.
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation
anticorps