Alternative Name
Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340; ERBB2; HER2; MLN19; NEU; NGL
Immunogen
Synthetic peptide, corresponding to amino acids 641-690 of Human ErbB2/HER2.
Swiss-Prot
P04626
Reactivity
Human,Mouse,Rat
Applications
WB IHC IF
Application_all
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity
ErbB2/HER2 (K676) polyclonal antibody detects endogenous levels of ErbB2/HER2 protein.
Usage
Western blot (WB) analysis of ErbB2/HER2 (K676) pAb at 1:500 dilution Lane1:3T3-L1 whole cell lysate(40ug) Lane2:Beas-2B whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:A375 whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(20ug)
BiowMW
~ 138,185 kDa
Note
For research use only, not for use in diagnostic procedure.
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation
anticorps