Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity
BDNF polyclonal antibody detects endogenous levels of BDNF protein.
Usage
Western blot (WB) analysis of BDNF polyclonal antibody at 1:1000 dilution Lane1:A549 whole cell lysate(40ug) Lane2:Hcc827 whole cell lysate(40ug) Lane3:The brain tissue lysate of Mouse(40ug) Lane4:The brain tissue lysate of Rat(40ug)
BiowMW
~ 27 kDa
Note
For research use only, not for use in diagnostic procedure.
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
