Alternative Name
Merlin; Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomerlin; Schwannomin; NF2; SCH
Immunogen
Synthetic phosphopeptide derived from human Merlin around the phosphorylation site of Serine 518.
Swiss-Prot
P35240
Reactivity
Human,Mouse,Rat
Applications
WB IF
Application_all
WB: 1:500~1:1000 IF: 1:50~1:200
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity
p-NF2/Merlin (S518) polyclonal antibody detects endogenous levels of NF2/Merlin protein only when phosphorylated at Ser518
Usage
Western blot (WB) analysis of p-NF2 (S518) pAb at 1:500 dilution Lane1:A375 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:The Uterus tissue lysate of Rat(40ug) Lane4:CT26 whole cell lysate(40ug)
BiowMW
~ 70 kDa
Note
For research use only, not for use in diagnostic procedure.
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation
anticorps