Long name
Neuro D2 Polyclonal Antibody, Biotin Conjugated
Category
Conjugated Primary Antibodies
Conjugation
Biotin
Host Organism
Rabbit (Oryctolagus cuniculus)
Target Antigen
Neuro D2
Specificity
This is a highly specific antibody against Neuro D2.
Modification
Unmodified
Modification site
None
Clonality
Polyclonal
Clone
Polyclonal antibody
Concentration
1ug per 1ul
Source
KLH conjugated synthetic peptide derived from human Neuro D2
Tested applications
WB, IHC-P
Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500)
Crossreactivity
Human, Mouse, Rat
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Antigen background
Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes, both of which work together to activate DNA transcription. Class A proteins include the ubiquitously expressed E-box binding factors, namely E2A, ITF-2 and HEB, while class B proteins, such as MyoD, myogenin and Neuro D (BETA2), are transiently expressed and exhibit a much more limited tissue distribution. Working in opposition to these positively acting factors are a specialized group of basic helix-loop-helix (bHLH) transcription factors that function as dominant negative regulators and are involved in cell lineage determination and differentiation. Neuro D2 (neurogenic differentiation 2), also known as NDRF, NEUROD2 or bHLHa1, is a 382 amino acid nuclear protein that contains one bHLH domain and functions to induce neurogenic differentiation, playing an important role in the maintenance and determination of cell fate.
Purification method
This antibody was purified via Protein A.
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Excitation Emission
N/A
Synonyms
bHLHa1; Class A basic helix-loop-helix protein 1; class A basic helix loop helix protein 1; NDF2_HUMAN; NDR2; NDRF; Neuro-D2; NeuroD-related factor; NeuroD2; Neurogenic dferentiation factor 2; NDF2_HUMAN; neuroD related factor; NeuroD2; neurogenic basic helix loop helix protein; neurogenic dferentiation 2; Neurogenic dferentiation factor 2; neuronal dferentiation 2.
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Additional conjugation
Biotinylated
French translation
anticorps