Long name
Activated Notch1 Polyclonal Antibody, Cy5.5 Conjugated
Category
Conjugated Primary Antibodies
Conjugation
Cy5.5
Host Organism
Rabbit (Oryctolagus cuniculus)
Target Antigen
Neurogenic locus notch homolog protein 1
Specificity
This is a highly specific antibody against Neurogenic locus notch homolog protein 1.
Modification
Unmodified
Modification site
None
Clonality
Polyclonal
Clone
Polyclonal antibody
Concentration
1ug per 1ul
Immunogen_range
1754-1780/2555
Source
KLH conjugated synthetic peptide derived from human Notch 1 intracellular domain.
Gene ID number
4851
Swiss Prot
P46531
Tested applications
IF(IHC-P)
Recommended dilutions
IF(IHC-P)(1:50-200)
Crossreactivity
Human
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Antigen background
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).
Purification method
This antibody was purified via Protein A.
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Excitation Emission
675nm/694nm
Synonyms
hN1; AOS5; TAN1; AOVD1; Neurogenic locus notch homolog protein 1; Notch 1; Translocation-associated notch protein TAN-1; NOTCH1
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.
Additional conjugation
Cy5.5,Cy5.5
Conjugated
These antibodies are excite for emission at 678nm and detected at a 694 nm wavelengths.
French translation
anticorps