NGB/neuroglobin Antibody, Biotin Conjugated

Basic informations

  • Size: 0.1ml
  • Catalog number: bs-1859R-Biotin
  • Price: 333.00EUR
NGB/neuroglobin Antibody, Biotin Conjugated

Long name

NGB/neuroglobin Polyclonal Antibody, Biotin Conjugated

Category

Conjugated Primary Antibodies

Conjugation

Biotin

Host Organism

Rabbit (Oryctolagus cuniculus)

Target Antigen

NGB/neuroglobin

Specificity

This is a highly specific antibody against NGB/neuroglobin.

Modification

Unmodified

Modification site

None

Clonality

Polyclonal

Clone

Polyclonal antibody

Concentration

1ug per 1ul

Immunogen_range

100-150/150

Subcellular location

Cytoplasm, Mitochondrion

Source

KLH conjugated synthetic peptide derived from mouse NGB

Gene ID number

64242

Swiss Prot

Q9ER97

Tested applications

WB, IHC-P

Recommended dilutions

WB(1:100-1000), IHC-P(1:100-500)

Crossreactivity

Human, Mouse, Rat

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Antigen background

Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.

Purification method

This antibody was purified via Protein A.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Excitation Emission

N/A

Synonyms

Neuroglobin; Ngb

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Additional conjugation

Biotinylated

French translation

anticorps