Long name
NGB/neuroglobin Polyclonal Antibody, Biotin Conjugated
Category
Conjugated Primary Antibodies
Conjugation
Biotin
Host Organism
Rabbit (Oryctolagus cuniculus)
Target Antigen
NGB/neuroglobin
Specificity
This is a highly specific antibody against NGB/neuroglobin.
Modification
Unmodified
Modification site
None
Clonality
Polyclonal
Clone
Polyclonal antibody
Concentration
1ug per 1ul
Immunogen_range
100-150/150
Subcellular location
Cytoplasm, Mitochondrion
Source
KLH conjugated synthetic peptide derived from mouse NGB
Gene ID number
64242
Swiss Prot
Q9ER97
Tested applications
WB, IHC-P
Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500)
Crossreactivity
Human, Mouse, Rat
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Antigen background
Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.
Purification method
This antibody was purified via Protein A.
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Excitation Emission
N/A
Synonyms
Neuroglobin; Ngb
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Additional conjugation
Biotinylated
French translation
anticorps