NPS/Neuropeptide S Antibody, FITC Conjugated

Basic informations

  • Size: 0.1ml
  • Catalog number: bs-11422R-FITC
  • Price: 333.00EUR
NPS/Neuropeptide S Antibody, FITC Conjugated

Long name

NPS/Neuropeptide S Polyclonal Antibody, FITC Conjugated

Category

Conjugated Primary Antibodies

Conjugation

FITC

Host Organism

Rabbit (Oryctolagus cuniculus)

Target Antigen

NPS/Neuropeptide S

Specificity

This is a highly specific antibody against NPS/Neuropeptide S.

Modification

Unmodified

Modification site

None

Clonality

Polyclonal

Clone

Polyclonal antibody

Concentration

1ug per 1ul

Source

KLH conjugated synthetic peptide derived from human Neuropeptide S

Gene ID number

594857

Tested applications

IF(IHC-P)

Recommended dilutions

IF(IHC-P)(1:50-200)

Crossreactivity

Human

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Antigen background

Neuropeptides are regulators of synaptic transmission and their effects are mediated by G-protein coupled receptors. NPS (Neuropeptide S) is a 20 amino acid peptide cleaved from a larger precursor that contains a hydrophobic signal peptide and proteolytic cleavage processing sites. The N-terminal residue of NPS is always a serine regardless of the species. NPS is predominantly found in the central nervous system and plays an important role regulating sleep/wake functions, locomotion, arousal/anxiety responses and food intake. NPS functions by binding and activating its receptor, NPSR, and increasing intracellular calcium levels thereby acting as an excitatory transmitter. In addition, NPS stimulates the hypothalamo-pituitary adrenal (HPA) axis via the release of corticotropin-releasing factor (CRF) and arginine vasopressin (AVP). NPS and its receptor NPSR may also play a role in asthma pathogenesis.

Purification method

This antibody was purified via Protein A.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Excitation Emission

494nm/518nm

Synonyms

NeuropeptideS; Neuropeptide-S; NPS; NPS_HUMAN.

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.

Additional conjugation

Anti-FITC Antibody

French translation

anticorps