Long name
RNF131 Polyclonal Antibody, Cy3 Conjugated
Category
Conjugated Primary Antibodies
Conjugation
Cy3
Host Organism
Rabbit (Oryctolagus cuniculus)
Target Antigen
RNF131
Specificity
This is a highly specific antibody against RNF131.
Modification
Unmodified
Modification site
None
Clonality
Polyclonal
Clone
Polyclonal antibody
Concentration
1ug per 1ul
Source
KLH conjugated synthetic peptide derived from human PJA2/RNF131
Gene ID number
9867
Tested applications
IF(IHC-P)
Recommended dilutions
IF(IHC-P)(1:50-200)
Crossreactivity
Human, Mouse, Rat
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Antigen background
PJA2, also known as E3 ubiquitin-protein ligase praja-2, RNF131 (ring finger protein 131) or Neurodap1, is a 708 amino acid protein that contains one ring-type zinc finger and exists as two alternatively spliced isoforms. Significantly conserved in chimpanzee, dog, cow, mouse, rat, chicken and zebrafish, PJA2 shares 52% identity with PJA1, which is involved in protein ubiquitination in brain and may play a role in X-linked mental retardation. Encoded by a gene that maps to human chromosome 5q21.3, PJA2 localizes to both endoplasmic reticulum and Golgi apparatus membranes. Participating in E2-dependent, E3 ubiquitin-protein ligase activity, PJA2 binds to a variety of E2s and interacts with ubiquitin-conjugating enzymes, such as UBE2D2, in vitro.
Purification method
This antibody was purified via Protein A.
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Excitation Emission
512,550nm/570,615nm
Synonyms
E3 ubiquitin protein ligase Praja2; E3 ubiquitin-protein ligase Praja-2; KIAA0438; Neurodap 1; Neurodap1; PJA 2; PJA2; PJA2_HUMAN; Praja 2; Praja 2 RING H2 mot containing; praja ring finger 2; Praja ring finger 2 E3 ubiquitin protein ligase; Praja2; RING finger protein 131; RNF 131; RNF131.
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-RNF131 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
Additional conjugation
cy3 conjugation kit
French translation
anticorps