Neuron-Specific Enolase
neuron-specific enolase; Gamma-enolase; gamma-enolase; neural enolase; neuron-specific enolase; 2-phospho-D-glycerate hydro-lyase; enolase 2, gamma, neuronal; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE
Antibodies
Mnoclonal antibodies
NSE
N/A
Eno2; Eno2; NSE; RNEN3; Eno-2; NSE
Monoclonal
IgG2a for MAbs 5E2, 1C1, 5A4, H11 and 2F1 IgG2b for MAb 5G10
5G10, 5E2, 5A4, 1C1, H11, 2F1
N/A
N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Human NSE; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Chromatography on protein G Sepharose
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
N/A
Store the antibody at +4 degrees Celsius.
ELISA (EIA)
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Mus musculus