Also known as
Neuron Specific Nuclear Protein
Other names
N/A
Category
Antibodies
Subcategory
Mnoclonal antibodies
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Clonality
Monoclonal
Immunoglobulin isotype
IgG1
Clone
3H412
Host organism
Mouse (Mus musculus)
Source organism
Mouse (Mus musculus)
Species reactivity
Mouse (Mus musculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
Recognizes mouse Neuron Specific Nuclear Protein (NeuN). Reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. Non-reactive cell types include Purkinje, mitral and photoreceptor cells. Species Crossreactivity: rat ; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Purification method
Affinity Purified by Protein A affinity chromatography.
Form/Appearance
Supplied as a liquid in PBS, pH 7.1, 15mg/ml BSA, 0.1% sodium azide. Labeled with Biotin.
Concentration
N/A
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Tested applications:
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
French translation
anticorps