Also known as
NEUROFILAMENT H
Other names
Neurofilament heavy polypeptide; Neurofilament heavy polypeptide; neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; neurofilament triplet H protein; neurofilament, heavy polypeptide; 200 kDa neurofilament protein; Neurofilament triplet H protein
Category
Antibodies
Subcategory
Mnoclonal antibodies
Gene name
Nefh; Nefh; Nfh; Nfh; NF-H
Gene name synonims
Nefh; Nefh; Nfh; Nfh; NF-H
Other gene names
Nefh; Nefh; Nfh; Nfh; NF-H
Clonality
Monoclonal
Immunoglobulin isotype
IgG1
Clone
RT97
Host organism
Mouse (Mus musculus)
Species reactivity
Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
NEUROFILAMENT H This item recognises the 200kD neurofilament protein in a range of species. The antibody stains various tumours including phaeochromocytoma, paraganglioma and ganglioneuroblastoma. It has also been demonstrated that the RT97 antibody recognises a phosphorylation dependent epitope on microtubule associated protein 1B (MAP1B) and on Tau in Western blots. Reactivity of the antibody with MAP1 and Tau is not seen under the conditions for immunohistochemistry.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Purification method
N/A
Form/Appearance
Purified (Purified IgG - liquid)
Concentration
IgG concentration 1.0 mg/ml
Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost free freezers is not recommended. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
Tested applications:
Immunohistology - Paraffin (IHC), Immunohistology - Frozen (IHC)
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus,Rattus norvegicus
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
French translation
anticorps