Long name
NGB/neuroglobin Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
Also known as
Anti-NGB/neuroglobin PAb ALEXA FLUOR 488
Category
Conjugated Primary Antibodies
Conjugated with
ALEXA FLUOR® 488
Host Organism
Rabbit (Oryctolagus cuniculus)
Target Antigen
NGB/neuroglobin
Specificity
This is a highly specific antibody against NGB/neuroglobin.
Modification
Unmodified
Modification Site
None
Clonality
Polyclonal
Clone
Polyclonal antibody
Concentration
1ug per 1ul
Immunogen range
100-150/150
Subcellular location
Cytoplasm, Mitochondrion
Source
KLH conjugated synthetic peptide derived from mouse NGB
Gene ID Number
64242
Swiss Prot
Q9ER97
Tested applications
IF(IHC-P)
Recommended dilutions
IF(IHC-P)(1:50-200)
Crossreactivity
Human, Mouse, Rat
Cross-reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigen
Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.
Purification
Purified by Protein A.
Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission
499nm/519nm
Synonyms
Neuroglobin; Ngb
Properties
For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-NGB/neuroglobin has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Conjugation
Alexa Fluor
French translation
anticorps