Long name
NPS/Neuropeptide S Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
Also known as
Anti-NPS/Neuropeptide S PAb ALEXA FLUOR 488
Category
Conjugated Primary Antibodies
Conjugated with
ALEXA FLUOR® 488
Host Organism
Rabbit (Oryctolagus cuniculus)
Target Antigen
NPS/Neuropeptide S
Specificity
This is a highly specific antibody against NPS/Neuropeptide S.
Modification
Unmodified
Modification Site
None
Clonality
Polyclonal
Clone
Polyclonal antibody
Concentration
1ug per 1ul
Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Neuropeptide S
Gene ID Number
594857
Tested applications
IF(IHC-P)
Recommended dilutions
IF(IHC-P)(1:50-200)
Crossreactivity
Human
Cross-reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigen
Neuropeptides are regulators of synaptic transmission and their effects are mediated by G-protein coupled receptors. NPS (Neuropeptide S) is a 20 amino acid peptide cleaved from a larger precursor that contains a hydrophobic signal peptide and proteolytic cleavage processing sites. The N-terminal residue of NPS is always a serine regardless of the species. NPS is predominantly found in the central nervous system and plays an important role regulating sleep/wake functions, locomotion, arousal/anxiety responses and food intake. NPS functions by binding and activating its receptor, NPSR, and increasing intracellular calcium levels thereby acting as an excitatory transmitter. In addition, NPS stimulates the hypothalamo-pituitary adrenal (HPA) axis via the release of corticotropin-releasing factor (CRF) and arginine vasopressin (AVP). NPS and its receptor NPSR may also play a role in asthma pathogenesis.
Purification
Purified by Protein A.
Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission
499nm/519nm
Synonyms
NeuropeptideS; Neuropeptide-S; NPS; NPS_HUMAN.
Properties
For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-NPS/Neuropeptide S has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Conjugation
Alexa Fluor
French translation
anticorps