Rabbit Ciliary Neurotrophic Factor (CNTF) ELISA Kit, 96 well plate

Basic informations

  • Size: 96T
  • Catalog number: EKU03195
  • Price: 831.00EUR
Rabbit Ciliary Neurotrophic Factor (CNTF) ELISA Kit, 96 well plate

Verified reactivity

Oryctolagus cuniculus (Rabbit)

Detection limits

31.25-2000 pg/mL

Sensitivity limit

11,8 pg/mL

Samples to be analyzed

Serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Assay duration

3 hours

Verified applications

ELISA

Assay class

Sandwich

ELISA detection

Colorimetric

Protein number

P14188

Alternate protein number

Please refer to SwissProt

Gene number

100009117

Gene name

Ciliary Neurotrophic Factor

Alternate gene name

HCNTF

Estimated production time

7-11 business days

Shipping requirements

Blue ice

Storage recommendation

-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Use for

8 months

ELISA's specificity

This assay has high sensitivity and excellent specificity for detection of Ciliary Neurotrophic Factor (CNTF). No significant cross-reactivity or interference between Ciliary Neurotrophic Factor (CNTF) and analogues was observed.

ELISA's cross-reactivity

This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.

Assay principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ciliary Neurotrophic Factor (CNTF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ciliary Neurotrophic Factor (CNTF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ciliary Neurotrophic Factor (CNTF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ciliary Neurotrophic Factor (CNTF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Protocol

Please see ELISA's datasheet, otherwise contact us

Precision of the test

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ciliary Neurotrophic Factor (CNTF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ciliary Neurotrophic Factor (CNTF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV

ELISA's stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

QC

The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Research main area

Cytokine;Neuro science;

Warnings

The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Notes

For research use only. Not for diagnostic procedures.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Description

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.

About

Rabbits are used for polyclonal antibody production by bioma. Rabbit antibodies are very stable and can be stored for several days at room temperature. bioma adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Latin name

Oryctolagus cuniculus