Human NEF3 (Neurofilament 3) CLIA Kit

Basic informations

  • Size: 96Test
  • Catalog number: E-CL-H0535-96T
  • Price: 541.00EUR
Human NEF3 (Neurofilament 3) CLIA Kit

Detection Type

Colormetric

Target Species

Human

Target Synonym

NEFM, NF-M, NFM, Neurofilament, medium polypeptide

Test principle

This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NEF3 . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NEF3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NEF3 , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NEF3 . You can calculate the concentration of Human NEF3 in the samples by comparing the RLU value of the samples to the standard curve.

Sensitivity

7.5pg/mL

Detection Range

12.5~800pg/mL

Target Name

NEF3

UNIProt ID

NA

Tested Sample Types

Serum, Plasma, Cell supernatant

Product Name

Human NEF3 (Neurofilament 3) CLIA Kit

Type

Sandwich

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.