Detection Type
Colormetric
Target Species
Mouse
Target Synonym
NP-Y, PYY4
Test principle
This EIA kit uses the Competitive-EIA principle. The micro EIA plate provided in this kit has been pre-coated with Mouse NPY. During the reaction, Mouse NPY in the sample or standard competes with a fixed amount of Mouse NPY on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse NPY. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse NPY in the samples is then determined by comparing the OD of the samples to the standard curve.
Sensitivity
18.75 pg/mL
Detection Range
31.25~2000pg/mL
Target Name
NPY
UNIProt ID
P57774
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Mouse NPY(Neuropeptide Y) EIA Kit
Type
Competitive
Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus