Detection Type
Colormetric
Target Species
Rat
Target Synonym
NA
Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat NEFH. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NEFH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NEFH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NEFH. You can calculate the concentration of Rat NEFH in the samples by comparing the OD of the samples to the standard curve.
Sensitivity
18.75pg/mL
Detection Range
31.25~2000pg/mL
Target Name
NEFH
UNIProt ID
P16884
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Rat NEFH(Neurofilament,HeavyPolypeptide) EIAKit
Type
Sandwich
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus