Synonym
Neurocalcin-Delta; NCALD
Activity
NA
Sequence
Met1-Phe193
Fusion tag
N-6His
Accession
P61601
Expressed Host
E.coli
Shipping
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Purity
＞90% as determined by reducing SDS-PAGE.
Endotoxin
＜1.0 EU per µg as determined by LAL test.
Stability and Storage
Store at < -20℃, stable for 6 monthshs after receipt.Please minimize freeze-thaw cycles
Mol Mass
24.4 kDa
AP Mol Mass
20 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 100mM NaCl, 1mM DTT, 40% Glycerol, pH 8.0.
Reconstitution
Please refer to it for detailed information.
Background
Neurocalcin-delta (NCALD) is a neuronal calcium-binding protein that belongs to the neuronal calcium sensor (NCS) family. It expressed in mammalian brains. NCALD contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein possesses a Ca2+ /myristoyl switch. It is cytosolic at resting calcium levels. However, elevated intracellular calcium levels induce a conformational change which exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. NCALD protein is thought to be a regulator of G protein-coupled receptor signal transduction.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Source
Recombinants or rec. proteins
Group
recombinants