Recombinant Human Neuropilin 2 / NRP2 Protein (Fc tag)

Basic informations

  • Size: 20µg
  • Catalog number: PKSH031480-20µg
  • Price: 356.00EUR
Recombinant Human Neuropilin 2 / NRP2 Protein (Fc tag)

Synonym

Neuropilin-2;NP2;NPN2;PRO2714;VEGF165R2

Activity

Measured by its binding ability in a functional ELISA.2.Immobilized human VEGFC-His (Cat:10542-H08H) at 10μg/mL (100μL/well) can bind human NRP2-Fc (Cat:10695-H02H), the EC50 of human NRP2-Fc is 0.1-0.5μg/mL.

Sequence

Met1-Tyr855

Fusion tag

C-Fc

Accession

NP_003863.2

Expressed Host

HEK293 Cells

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Purity

>95 % as determined by SDS-PAGE

Endotoxin

<1.0 EU per µg as determined by the LAL method

Stability and Storage

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Mol Mass

120.7 kDa

AP Mol Mass

119-129 kDa

Formulation

Lyophilized from sterile PBS, pH 7.4

Background

Neuropilin-2 (NRP-2) which is related to NRP-1, is a type I? transmembrane glycoprotein and has the structure characteristic with five main extracellular domains: two complement binding (CUB) domains, two coagulation factor V/VIII homology domains, and a MAM (meprin, tyrosine phosphatase domain) region. NRP-2 is a receptor capable of binding two disparate ligands, classⅢ semaphorins (SEMA) and vascular endothelial growth factors (VEGF), and thus regulates two diverse systems by activating cellular signaling pathways via interacting with other cell surface receptors such as VEGF receptors and plexins. NRP-2 is well known for its role in facilitating axonal guidance during the development of the neuronal system, and additionally, it is also expressed in vascular endothelial cells and lymphatic endothelium where it affects proliferation, migration, angiogenesis, as well as formation of small lymphatic vessels and capillaries. Recent study has identified NRP-2 as a polysialylated protein expressed in human dendritic cells and modulates DC-T cell Interactions. Nearly all tumor cells express neuropilins and NRP-2 is predominantly expressed in neuronal tumors and melanomas. Furthermore, it is suggested that as the specific ligand for NRP-2, SEMA 3F inhibits tumor angiogenesis and metastasis.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Source

Recombinants or rec. proteins

Group

recombinants