6-11 business days
NA
HumanngB
Human
Serum, Plasma, Biological Fluids
0.16-10 ng/mL
0.1 ng/mL
NA
Colorimetric
4.5 hours
For research use only. Not for diagnostic procedures.
Sandwich
Ice packs
Short term: 4°C; Long term: see manual.
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Use HumanngB (Neuroglobin) ELISA Kit before 6 months
NA
No significant cross-reactivity or interference was observed.
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HumanngB . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HumanngB and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HumanngB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of HumanngB. The concentration of HumanngB in samples can be calculated by comparing the OD of the samples to the standard curve.
NA
The kit is manufactured at ISO 9001 certified facilities.
Cancer, Neuroscience
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED